000			05152cam a22004577i 4500
001			17966595
005			20160317120906.0
008			131209t20142014nyua b 001 0 eng d
010			_a 2013957132
016	7		_a016631504 _2Uk
020			_a9781627037662 (hb)
035			_a(OCoLC)ocn859042349
040			_aYDXCP _beng _cYDXCP _erda _dBTCTA _dVXG _dUKMGB _dIDL _dSTF _dDEBBG _dOCLCO _dNUI _dOCLCQ _dIPL _dDLC
042			_alccopycat
050	0	0	_aQK95.6 _b.M63 2014
082	0	0	_a582 BES/Mol _223
245	0	0	_aMolecular plant taxonomy :methods and protocols _cedited by Pascale Besse, UMR C53 PVBMT Université de la Réunion - Cirad, Université de la Réunion, Ile de la Réunion, France.
260			_aNew York: _bHumana Press; _c2014
300			_axii, 402 pages : _billustrations (some color) ; _c27 cm.
490	1		_aMethods in molecular biology, _x1064-3745 ; _v1115
490	1		_aSpringer protocols, _x1949-2448
504			_aIncludes bibliographical references and index.
505	0		_aPlant taxonomy : an historical perspective, current challenges and perspectives / Germinal Rouhan, Myriam Gaudeul -- Guidelines for the choice of sequences for molecular plant taxonomy / Pascale Besse -- Leaf tissue sampling and DNA extraction protocols / Kassa Semagn -- DNA extraction from herbarium specimens / Lenka Záveská Drábková -- Analysis of variation in chloroplast DNA sequences / Berthold Heinze, Agnieszka Koziel-Monte, Daniela Jahn -- Mitochondrial genome and plant taxonomy / Jérôme Duminil -- Nuclear ribosomal RNA genes : ITS region / Pascale Besse -- New technologies for ultrahigh-throughput genotyping in plant taxonomy / David Edwards [and three others] -- Development of microsatellite-enriched libraries / Hélène Vignes, Ronan Rivallan -- Randomly amplified polymorphic DNA (RAPD) and derived techniques / Kantipudi Nirmal Babu [and six others] -- Multilocus profiling with AFLP, ISSR and SAMPL / Luis F. Goulao, Cristina M. Oliveira -- Transposon-based tagging : IRAP, REMAP, and iPBS / Ruslan Kalendar, Alan H. Schulman -- Phylogenetic reconstruction methods : an overview / Alexandre De Bruyn, Darren P. Martin, Pierre Lefeuvre -- Application of flow cytometry for estimating genome size and ploidy level in plants / Jaume Pellicer, Ilia J. Leitch -- Molecular cytogenetics (FISH and fluorochrome banding) : resolving species relationships and genome organization / Sonja Siljak-Yakovlev [and three others] -- GISH : resolving interspecific and intergeneric hybrids / Nathalie Piperidis -- On the relevance of molecular tools for taxonomic revision in Malvales, Malvaceae s.l., and Dombeyoideae / Timothée Le Péchon, Luc D.B. Gigord -- What has molecular systematics contributed to our knowledge of the plant family Proteaceae? / Peter H. Weston.
520			_aPlant taxonomy is an ancient discipline facing new challenges with the current availability of a vast array of molecular approaches which allow reliable genealogy-based classifications. Although the primary focus of plant taxonomy is on the delimitation of species, molecular approaches also provide a better understanding of evolutionary processes, a particularly important issue for some taxonomic complex groups. 'Molecular Plant Taxonomy: Methods and Protocols" describes laboratory protocols based on the use of nucleic acids and chromosomes for plant taxonomy, as well as guidelines for phylogenetic analysis of molecular data. Experts in the field also contribute review and application chapters that will encourage the reader to develop an integrative taxonomy approach, combining nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written in the successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Molecular Plant Taxonomy: Methods and Protocols seeks toprovide conceptual as well as technical guidelines to plant taxonomists and geneticists.--
650		0	_aPlants _xClassification _xMolecular aspects.
650	1	2	_aPlants _xclassification _vLaboratory Manuals.
650	2	2	_aPlants _xgenetics _vLaboratory Manuals.
650	2	2	_aPhylogeny _vLaboratory Manuals.
650	0	7	_aMolekularbiologie. _2gnd
650	0	7	_aPflanzen. _2gnd
650	0	7	_aSystematik. _2gnd
655		2	_aLaboratory Manuals.
655		4	_aAufsatzsammlung.
700	1		_aBesse, Pascale _eeditor.
710	2		_aSpringer Science+Business Media, _ecopyright holder.
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 1115.
830		0	_aSpringer protocols (Series)
906			_a7 _bcbc _ccopycat _d2 _eepcn _f20 _gy-gencatlg
955			_brl09 2014-06-27 z-processor _irl09 2014-07-23 ; to Dewey _atd11 2014-12-29 typo corrected in 245c
955			_apc17 2013-12-09
999			_c119855 _d119855